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Immunologie und Infektion
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Single-Copy Gene Expression for Characterizing Multidrug Efflux Systems
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Electroporation Procedure for the Single-Copy Gene Expression in an Efflux-Deficient Bacterial Strain
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Immunologie und Infektion
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JoVE Journal Immunologie und Infektion
Electroporation Procedure for the Single-Copy Gene Expression in an Efflux-Deficient Bacterial Strain

Electroporation Procedure for the Single-Copy Gene Expression in an Efflux-Deficient Bacterial Strain

Single-Copy Gene Expression for Characterizing Multidrug Efflux Systems

DOI:
10.3791/201442-v

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03:04 min

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January 05, 2024

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,
1Department of Microbiology,University of Manitoba

Tags

ElectroporationPTNS2 Helper PlasmidPUC18-Tmini-Tn7-Tlac Gentamicin AdeIJK Insertion PlasmidElectrocompetent CellsLB BrothLB AgarGentamicinColony PCRABglmS2 ForwardTn7 ReverseA.baumannii ATCC 17978AB 258

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