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在哺乳动物细胞的RNAi筛选使命esiRNA
JoVE Revista
Biología
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JoVE Revista Biología
MISSION esiRNA for RNAi Screening in Mammalian Cells
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在哺乳动物细胞的RNAi筛选使命esiRNA

15,875 Views

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15:31 min

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May 12, 2010

DOI:

10.3791/2008-v

DOI:
10.3791/2008-v

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15:31 min
May 12, 2010

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3 Views
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1Max Planck Institute of Molecular Cell Biology and Genetics

Tags

MISSION EsiRNARNAi ScreeningMammalian CellsGene Expression ControlSmall Interfering RNAs (siRNAs)DicerRNA-induced Silencing Complex (RISC)Target MRNA CleavageLoss Of Gene Function StudiesChemical Synthesis Of SiRNAsEndonucleolytic CleavageTarget-specific Long Double-stranded RNAsRNase IIIEndoribonuclease-prepared SiRNAEfficacy Of SiRNAs And EsiRNAsOff-target Effects

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Theis, M., Buchholz, F. MISSION esiRNA for RNAi Screening in Mammalian Cells. J. Vis. Exp. (39), e2008, doi:10.3791/2008 (2010).

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