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Aislamiento de pequeñas vesículas extracelulares (sEV) mediante ultracentrifugación diferencial

JoVE Revista
Bioquímica

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JoVE Revista Bioquímica

Isolating Small Extracellular Vesicles (sEVs) by Differential Ultracentrifugation

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Aislamiento de pequeñas vesículas extracelulares (sEV) mediante ultracentrifugación diferencial

403 Views

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04:25 min

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June 30, 2023

DOI:

10.3791/200347-v

DOI:

10.3791/200347-v

•

04:25 min

June 30, 2023

•

4 Views

Jiale Cheng², Jiong Zhu², Yujiao Liu³, Chen Yang³, Yuehui Zhang, Yuchen Liu, Chaozhi Jin, Jian Wang²

¹School of Basic Medical Sciences,Anhui Medical University, ²State Key Laboratory of Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences (Beijing),Beijing Institute of Lifeomics, ³School of Life Sciences,Hebei University

Tags

Extracellular Vesicles SEVs Differential Ultracentrifugation Macrophages FBS DMEM PBS Centrifugation Ultracentrifugation Pellets Supernatant Debris Microvesicles Protein Preparation

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