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The Importance of Correct Protein Concentration for Kinetics and Affinity Determination in Structure-function Analysis
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March 17, 2010
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Protein ConcentrationKineticsAffinity DeterminationStructure-function AnalysisCysteine Protease InhibitorCystatin BPapainReal-time Label-free AnalysisBiacore X100Point MutationsInteractionCalibration-free Concentration Analysis (CFCA)A280Single-cycle Kinetics (SCK)Association Rate Constant (ka)Affinity (KD)Reliable ResultsInteraction Mechanism
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Pol, E. The Importance of Correct Protein Concentration for Kinetics and Affinity Determination in Structure-function Analysis . J. Vis. Exp. (37), e1746, doi:10.3791/1746 (2010).
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