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1Department of Otology and Laryngology,Harvard Medical School, 2Eaton-Peabody Laboratory,Massachusetts Eye and Ear Infirmary, 3Department of Communication Sciences and Disorders,Emerson College, 4Program in Speech and Hearing Bioscience and Technology, Division of Health Science and Technology,Harvard
Capítulos
- 00:00Título
- 00:05Introduction
- 00:44Procedure Overview
- 01:45Day 1: Coating of Glass Coverslips and Heat Sterilization Tools
- 02:41Day 2: Isolation and Culture of the Murine Organ of Corti
- 08:15Applications for Isolated Organ of Corti
- 11:08Day 4: Visualization of Electroporated Cells
- 11:37Conclusion
Este procedimento descreve um método para o isolamento e cultura do órgão murino de Corti com ou sem o limbo espiral e neurônios do gânglio espiral. Nós também demonstrar um método para a expressão de um gene exógeno repórter no órgão de Corti explante por eletroporação.
Tags
Primary CulturePlasmid ElectroporationMurine Organ Of CortiSensory EpitheliumAuditionCochleaInner EarHair CellsMechanosensory CellsInner Hair CellsOuter Hair CellsBasilar MembraneSensorineural Hearing LossAuditory ResearchOrgan ExplantGene ExpressionStereocilia MotilityCell And Molecular BiologyHair Cell RegenerationIsolation And Culture MethodNeonatal MiceTemporal Bone Isolation
