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Dual Somatic Recordings from Gonadotropin-Releasing Hormone (GnRH) Neurons Identified by Green Fluorescent Protein (GFP) in Hypothalamic Slices
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Neurociencias
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JoVE Revista Neurociencias
Dual Somatic Recordings from Gonadotropin-Releasing Hormone (GnRH) Neurons Identified by Green Fluorescent Protein (GFP) in Hypothalamic Slices

Dual Somatic Recordings from Gonadotropin-Releasing Hormone (GnRH) Neurons Identified by Green Fluorescent Protein (GFP) in Hypothalamic Slices

DOI:
10.3791/1678-v

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09:30 min

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February 23, 2010

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,
1Department of Biology,University of Texas San Antonio – UTSA

Capítulos

  • 00:00Título
  • 00:36Introduction
  • 01:09Preparation of Hypothalamic Brain Slices
  • 03:12Preparation of Glass Pipettes
  • 03:57Current Clamp Recording
  • 07:25Validation of Cell-Attached Recording Method
  • 09:09Conclusion

Summary

Traducción Automática

Traducción Automática

Activity in neuronal systems often requires synchronous action potential discharges from neurons within a specific population. For example, pulses of gonadotropin-releasing hormone (GnRH) likely require coordinated activity between GnRH neurons. We present our methodological approach for reliably obtaining simultaneous electrophysiological recordings from the diffusely distributed GnRH neurons.

Tags

Gonadotropin-Releasing Hormone (GnRH)Green Fluorescent Protein (GFP)Hypothalamic SlicesPituitary ReleaseLuteinizing Hormone (LH)Follicle-stimulating Hormone (FSH)Reproductive FunctionHypothalamusMedian EminenceHypophysiotropic Portal SystemAnterior Pituitary GlandGonadotropinsSystemic CirculationEpisodic PulsesPeptide ContentReleasable PoolNeurosecretionSingle Unit ActivityMulti-unit RecordingsLH ReleaseGnRH SomataFibers

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