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由Stemgent人类TF慢病毒重新编程人类成纤维细胞生成诱导多能干细胞
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December 08, 2009
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我们展示了为生成诱导多能干的干细胞，人类体细胞用慢病毒介导的人为因素OCT4，SOX2，NANOG和Lin28交付协议。多能性证实了形态学和胚胎干（ES）细胞特异性标志物的存在。
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Induced Pluripotent Stem CellsReprogrammingHuman FibroblastsStemgent Human TF Lentivirus SetRetrovirus-mediated DeliveryOct4Sox2C-MycKlf4Pluripotent StateMouse FibroblastsHuman Somatic CellsIPS CellsTranscription FactorsRetroviral VectorsJames ThomsonNanogLin28ES CellsMorphologyProliferationDifferentiationPatient-specific IPS CellsPersonalized Regenerative Medicine Therapies
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Wu, D., Hamilton, B., Martin, C., Gao, Y., Ye, M., Yao, S. Generation of Induced Pluripotent Stem Cells by Reprogramming Human Fibroblasts with the Stemgent Human TF Lentivirus Set. J. Vis. Exp. (34), e1553, doi:10.3791/1553 (2009).
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