This content is Free Access.
Sistema Automatizado de Soltero mediciones de fluorescencia de la molécula de superficie inmovilizada Biomoléculas
12,635 Views
•
10:57 min
•
November 02, 2009
DOI:
Summary
Automatically generated
En este artículo se describe cómo obtener FRET rastros de moléculas individuales de ADN inmovilizado en una superficie utilizando un microscopio confocal de barrido automático.
Explore More Videos
Automated SystemSingle Molecule Fluorescence MeasurementsSurface-immobilized BiomoleculesFluorescence Resonance Energy Transfer MicroscopyFRET MicroscopyNucleic AcidsProteinsSm-FRET MeasurementsTime-tracesBiomolecular DynamicsStatistical AnalysesAutomated Scanning Confocal MicroscopeMicrofluidic CellBuffer ExchangeOxygen ContentConstant TemperatureMicrofluidic Device AssemblyDNA ImmobilizationBuffer PreparationPhotostabilityFluorophoresSetup Preparation
Read Article
Cite this Article
Di Fiori, N., Meller, A. Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules. J. Vis. Exp. (33), e1542, doi:10.3791/1542 (2009).
Copy