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Microinjection of Zebrafish Embryos to Analyze Gene Function
JoVE Revista
Biología
This content is Free Access.
JoVE Revista Biología
Microinjection of Zebrafish Embryos to Analyze Gene Function

Microinjection of Zebrafish Embryos to Analyze Gene Function

DOI:

07:18 min

March 09, 2009

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Capítulos

  • 00:00Título
  • 00:42Introduction
  • 01:12Zebrafish Egg Collection
  • 02:24Needle Pulling, Loading, and Preparation
  • 04:23Injection
  • 05:48Representative Results: Morpholino and mRNA Injection Phenotypes
  • 06:33Conclusion

Summary

Traducción Automática

This video shows how morpholino or mRNA can be injected into zebrafish embryos at the one-cell stage to decrease or increase the level of specific gene products during subsequent development.

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