Journal
/
Biología
/
Microcontact Printing of Proteins for Cell Biology
JoVE Revista
Biología
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Biología
Microcontact Printing of Proteins for Cell Biology

Microcontact Printing of Proteins for Cell Biology

DOI:
10.3791/1065-v

•

09:21 min

•

December 05, 2008

•

, ,
1Department of Biomedical Engineering,Columbia University

Capítulos

  • 00:00Título
  • 00:38Introduction
  • 01:22Microcontact Printing of Fibronectin onto Glass
  • 02:22Casting Stamps from the Topological Master
  • 04:22Microcontact Printing of Fibronectin onto Glass
  • 07:14Representative Results/Outcome
  • 08:55Conclusion

Summary

Traducción Automática

Traducción Automática

Microcontact printing is used extensively to pattern proteins and other molecules on material surfaces. We demonstrate the basic steps of this process, stamping patterns of fibronectin onto glass.

Tags

Microcontact PrintingProteinsCell BiologySpatial ComplexityExtracellular EnvironmentBiomoleculesMicroelectronicsMicrofabrication TechniquesPolydimethylsiloxane (PDMS) StampsFunctionalized ChemicalsMaterial SurfacesInnovative ApproachesPatterningFibronectinGlass CoverslipsTopological MasterSilicon WaferPhotoresistPhotolithographyFabrication Center

Article

Embed

Añadir a la lista de reproducción

Usage Statistics

Videos relacionados

A Gradient-generating Microfluidic Device for Cell Biology

Micro-scale Engineering for Cell Biology

Assessing Two-dimensional Crystallization Trials of Small Membrane Proteins for Structural Biology Studies by Electron Crystallography

Fluorescent Labeling of COS-7 Expressing SNAP-tag Fusion Proteins for Live Cell Imaging

Protein Membrane Overlay Assay: A Protocol to Test Interaction Between Soluble and Insoluble Proteins in vitro

Pull-down of Calmodulin-binding Proteins

Modified Yeast-Two-Hybrid System to Identify Proteins Interacting with the Growth Factor Progranulin

Cell Tracking Using Photoconvertible Proteins During Zebrafish Development

The Use of Chemostats in Microbial Systems Biology

Protocols for Implementing an Escherichia coli Based TX-TL Cell-Free Expression System for Synthetic Biology

Read Article