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Single Molecule Methoden zur Überwachung von Veränderungen in Bilayer Elastic Properties
JoVE Revista
Biología
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JoVE Revista Biología
Single Molecule Methods for Monitoring Changes in Bilayer Elastic Properties
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Single Molecule Methoden zur Überwachung von Veränderungen in Bilayer Elastic Properties

9,085 Views

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12:20 min

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November 03, 2008

DOI:

10.3791/1032-v

DOI:
10.3791/1032-v

•

12:20 min
November 03, 2008

•

2 Views
, , ,
1Department of Physiology and Biophysics,Weill Cornell Medical College, 2Department of Physiology and Biophysics,Weill Cornell Medical College of Cornell University

Summary

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Membranprotein-Funktion wird durch die Zellmembran Lipidzusammensetzung geregelt. Dieses Video-Artikel beschreibt, wie ein Pflaster mit Doppelschicht-Patch-Elektroden, sowie zur Verwendung von Gramicidin Kanäle als Reporter der veränderten Eigenschaften der Membran bilden.

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Single Molecule MethodsMonitoringChangesBilayer Elastic PropertiesMembrane Protein FunctionCell Membrane Lipid CompositionLipid-protein InteractionsLipid Bilayer-protein InteractionsPharmacological ResearchPharmaceuticalsLipid Bilayer Material PropertiesAltered Membrane Protein FunctionGramicidin ChannelsConformational ChangesGramicidin SubunitsProperties Of The LipidGramicidin Channel CurrentReporter Compounds

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Ingolfson, H., Kapoor, R., Collingwood, S. A., Sparre Andersen, O. Single Molecule Methods for Monitoring Changes in Bilayer Elastic Properties. J. Vis. Exp. (21), e1032, doi:10.3791/1032 (2008).

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