A Riboflavin and UV Light Treatment of Platelet Products to Eliminate Bacteria

Published: February 29, 2024

Abstract

Source: Keil, S. D., et al. Treatment of Platelet Products with Riboflavin and UV Light: Effectiveness Against High Titer Bacterial Contamination. J. Vis. Exp. (2015).

This video demonstrates the riboflavin and UV light-based pathogen reduction process against high-titer bacterial contamination in human platelet products. This process could help to lower the risk of severe adverse transfusion events associated with bacterial contamination.

Protocol

1. Platelet Product Preparation Collect a standard apheresis human platelet product at an accredited blood bank. The platelet product must meet the following collection specifications: 90–360 ml volume and 0.8 –3.8 x 106 platelets/µl concentration. Ensure that the platelet product has rested for a minimum of 2 hr prior to processing for pathogen reduction. Store the products at 22 ± 2 °C in a platelet incubator without agitation for these 2 hr…

Divulgaciones

The authors have nothing to disclose.

Materials

Mirasol Illuminator Terumo BCT N/A
Mirasol Treatment/Storage Bag Terumo BCT N/A
Hematology Analyzer Beckman-Coulter Ac•T diff
Blood Gas Analyzer Siemens RapidLab 1265
Sterile Docking Device Terumo BCT TSCD
Platelet Incubator Helmer PC2200
Orbital Incubator Shaker Lab-Line 4628
Dry Incubator Fisher Iso-temp
Class II A/B3 Biosafety Cabinet Thermo-forma 1286
Tubing Sealer Sebra Smart Sealer II
Table Top Centrifuge IEC Centra GP8R
10 ml Syringe BD 309604
30 ml Syringe BD 309650
5 ml Dilution Tube VWR 211-0058
50 ml Conical Tube Corning 430290
Micropipette Gilson P-200
Micropipette Rainin R-200
25 ml Disposable Serological Pipette Costar 4489

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A Riboflavin and UV Light Treatment of Platelet Products to Eliminate Bacteria. J. Vis. Exp. (Pending Publication), e21977, doi: (2024).

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