A Riboflavin and UV Light Treatment of Platelet Products to Eliminate Bacteria
Published: February 29, 2024
Abstract
Source: Keil, S. D., et al. Treatment of Platelet Products with Riboflavin and UV Light: Effectiveness Against High Titer Bacterial Contamination. J. Vis. Exp. (2015).
This video demonstrates the riboflavin and UV light-based pathogen reduction process against high-titer bacterial contamination in human platelet products. This process could help to lower the risk of severe adverse transfusion events associated with bacterial contamination.
Protocol
1. Platelet Product Preparation Collect a standard apheresis human platelet product at an accredited blood bank. The platelet product must meet the following collection specifications: 90–360 ml volume and 0.8 –3.8 x 106 platelets/µl concentration. Ensure that the platelet product has rested for a minimum of 2 hr prior to processing for pathogen reduction. Store the products at 22 ± 2 °C in a platelet incubator without agitation for these 2 hr…
Divulgaciones
The authors have nothing to disclose.
Materials
| Mirasol Illuminator | Terumo BCT | N/A | |
| Mirasol Treatment/Storage Bag | Terumo BCT | N/A | |
| Hematology Analyzer | Beckman-Coulter | Ac•T diff | |
| Blood Gas Analyzer | Siemens | RapidLab 1265 | |
| Sterile Docking Device | Terumo BCT | TSCD | |
| Platelet Incubator | Helmer | PC2200 | |
| Orbital Incubator Shaker | Lab-Line | 4628 | |
| Dry Incubator | Fisher | Iso-temp | |
| Class II A/B3 Biosafety Cabinet | Thermo-forma | 1286 | |
| Tubing Sealer | Sebra | Smart Sealer II | |
| Table Top Centrifuge | IEC | Centra GP8R | |
| 10 ml Syringe | BD | 309604 | |
| 30 ml Syringe | BD | 309650 | |
| 5 ml Dilution Tube | VWR | 211-0058 | |
| 50 ml Conical Tube | Corning | 430290 | |
| Micropipette | Gilson | P-200 | |
| Micropipette | Rainin | R-200 | |
| 25 ml Disposable Serological Pipette | Costar | 4489 |
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A Riboflavin and UV Light Treatment of Platelet Products to Eliminate Bacteria. J. Vis. Exp. (Pending Publication), e21977, doi: (2024).