Isolation and Culture of Bone Marrow Stromal Cells from a Mouse Tibia
Published: July 31, 2023
Abstract
Source: Maridas, D. E., et al. Isolation, Culture, and Differentiation of Bone Marrow Stromal Cells and Osteoclast Progenitors from Mice. J. Vis. Exp.(2018)
In this video, we demonstrate the isolation and culture of bone marrow stromal cells from the long bones of mice. The isolated adherent BMSCs can be used for further analysis.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Harvest of Bones from Mice Euthanize the animals using CO2 followed by cervical dislocation and spray them with 70% ethanol. NOTE: Ensure that animals of the same sex and preferably the same age are used. We routinely use animals aged 7 to 8 weeks. We recommend pooling cells from at le…
Divulgaciones
The authors have nothing to disclose.
Materials
| 200μL pipet tips | Rainin | 17014401 | Plasticware |
| 1.5mL centrifuge tubes | USA Scientific | 1615-5500 | Plasticware |
| 15mL conical tube | VWR | 89039-668 | Plasticware |
| 50mL conical tube | VWR | 89039-660 | Plasticware |
| Phosphate-Buffered Saline (PBS) | Sigma-Aldrich | 21-040-CM | Reagent |
| Ethanol | Fisher Science | 04-355-451 | Reagent |
| Dissection tools | |||
| 70μm filters | BD Falcon | 352350 | |
| 0.25% trypsin | Gibco | 25200 | Reagent |
| Kimwipe | VWR | 82003-820 |
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Isolation and Culture of Bone Marrow Stromal Cells from a Mouse Tibia. J. Vis. Exp. (Pending Publication), e21526, doi: (2023).