Seldinger Technique Based Pulmonary Artery Catheterization: A Procedure to Insert Pulmonary Artery Catheter Through the Jugular Vein in a Porcine Model
Published: April 30, 2023
Abstract
Source: Lyhne, M. D., et al. Closed Chest Biventricular Pressure-Volume Loop Recordings with Admittance Catheters in a Porcine Model J. Vis. Exp. (2021).
In this video, we have demonstrated a step-by-step procedure of pulmonary artery catheterization by inserting an intravascular catheter through jugular vein and reach the pulmonary artery to measure the cardiac chamber pressure in porcine model.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Intravascular accesses NOTE: Intravascular accesses are to be established in the right external jugular vein, the left external jugular vein, left carotid artery, left femoral artery, and right femoral vein. In the pig, the external jugular vein is much larger than the internal jugular vein and, th…
Representative Results
Figure 1: Intravascular access guided by ultrasound. (A) Ensure all equipment is ready, sterile, and well-functioning. Necessary equipment includes 7F sheaths (orange), 8F sheaths (blue), and a 12F sheath (white), guidewires for the Seldinger technique, venous catheters for intravascular access, syringe, isotonic saline, scalpel, and suture. (B) Use a linear ultr…
Divulgaciones
The authors have nothing to disclose.
Materials
| 12L-RS | GE Healthcare Japan | 5141337 | Ultrasound probe |
| Adhesive Aperature Drape (OneMed) | evercare | 1515-01 | 75 x 90 cm (hole: 6 x 8 cm) |
| Alaris GP Guardrails plus | CareFusion | 9002TIG01-G | Infusion pump |
| Alaris Infusion set | BD Plastipak | 60593 | |
| Alkoholswap | MEDIQ Danmark | 3340012 | 82% ethanol, 0,5% chlorhexidin, skin disinfection |
| Amplatz Support Wire Guide ExtraStiff | Cook Medical | THSF-25-260-AES | diameter: 0.025 inches, length: 260 cm |
| BD Connecta | BD | 394601 | Luer-Lock |
| BD Emerald | BD | 307736 | 10 mL syringe |
| BD Luer-Lock | BD Plastipak | 300865 | BD = Becton Dickinson, 50 mL syringe |
| BD Venflon Pro | Becton Dickinson Infusion Therapy | 393208 | 17G |
| Check-Flo Performer Introducer | Cook Medical | RCFW-16.0P-38-30-RB | 16 F sheath, 30 cm long |
| Cios Connect S/N 20015 | Siemens Healthineers | C-arm | |
| D-LCC12A-01 | GE Healthcare Finland | Pressure measurement monitor | |
| Fast-Cath Hemostasis Introducer 12F | St. Jude Medical | 406128 | L: 12 cm |
| Favorita II | Aesculap | Type: GT104 | |
| LabChart | ADInstruments | Data aquisition software | |
| Natriumklorid | Fresenius Kabi | 7340022100528 | 9 mg/ml Isotonic saline |
| PICO50 Aterial Blood Sampler | Radiometer | 956-552 | 2 mL |
| PowerLab 16/35 | ADInstruments | PL3516 | Serial number: 3516-1841 |
| Pressure Extension set | CODAN | 7,14,020 | Tube for anesthetics, 150 cm long, inner diameter 0.9 mm |
| Propolipid | Fresenius Kabi | 21636 | Propofol, 10 mg/mL |
| Fentanyl | B. Braun | 71036 | 50 mikrogram/mL |
| Radiofocus Introducer II | Radiofocus/Terumo | RS+B80N10MQ | 6+7+8F sheaths |
| S/5 Avance | Datex-Ohmeda | – | Mechanical ventilator |
| Safersonic Conti Plus & Safergel | SECMA medical innovation | SAF.612.18120.WG.SEC | 18 x 120 cm (Safersonic Sterile Transducer Cover with Adhesive Area and Safergel) |
| Scisense Catheter | Transonic Scisense | FDH-5018B-E245B | Serial number: 50-533. Pressurevolume catheter |
| Scisense Pressure-Volume Measurement System | Transonic Scisense | ADV500 Model: FY097B. | Pressure-volume box |
| Swan-Ganz CCOmbo | Edwards Lifesciences | 744F75 | 110 cm |
| TruWave Pressure Monitoring Set | Edwards Lifesciences | T434303A | 210 cm |
| Vivid iq | GE Medical Systems China | Vivid iq | |
| Zoletil 50 Vet (tiletamin 125 mg and zolazepam 125 mg) Butorphanol (10 mg/mL). Dose for pre-anesthesia: 10 mL/10 kg as intramuscular injection | Virbac | 83046805 | Zoletil Mix for pigs: 1 vial of Zoletil 50 Vet (dry matter); add 6.25 mL Xylozin (20 mg/mL), 1.25 mL ketamin (100 mg/mL) and 2.5 mL |
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Seldinger Technique Based Pulmonary Artery Catheterization: A Procedure to Insert Pulmonary Artery Catheter Through the Jugular Vein in a Porcine Model. J. Vis. Exp. (Pending Publication), e20830, doi: (2023).