Acute Biliary Pancreatitis Model: A Method to Generate Acute Biliary Pancreatitis Mouse Model via Pancreatic Duct Infusion
Published: April 30, 2023
Abstract
Source: Serra, M. B. et al. Sodium Taurocholate Induced Severe Acute Pancreatitis in C57BL/6 Mice. J. Vis. Exp. (2021)
This video describes the procedure for the generation of a mouse model with acute biliary pancreatitis via pancreatic infusion of sodium taurocholate. This model helps study pathophysiological changes induced by sodium taurocholate in the pancreas.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Laparotomy Anesthetize animals with xylazine (10 mg/kg) and ketamine solution (80 mg/kg), subcutaneously (0.1 mL/10g of body weight) using a 1 mL syringe and 13 x 0.45 mm needle 26 G ½. Check for sufficient anesthesia depth by pinching the toe. Control body temperature using heated pads. Ensure that all surgical ma…
Representative Results
Figure 1: Schematics of severe acute pancreatitis induction by 2.5% sodium taurocholate in C57BL/6 mice. (A) gallbladder; (B) common bile duct; (C) pancreatic duct; (D) portal vein; (E) microvessel clip; (F) puncture site (needle attached to a polyethylene tube an…
Divulgaciones
The authors have nothing to disclose.
Materials
| 0.4 mm needle | INTRAG MEDICAL TECH | 90183210 | 30G |
| 0.54 mm polyethylene tube | Tygon | 730010 | |
| Styrofoam block | |||
| Masking tape for mounting the mouse | Missner | 1236 | |
| Infusion pump scheduled to 10µL/min. | Havard aparatus-Peristaltic Pump Series | MA1 55-7766 | Model 66 Small Peristaltic |
| Scissors and forceps | |||
| Antiseptic providine iodine | Pfizer | 12086OR | Antisepsis |
| 70% ethanol | SIGMA | 459836 | Mix 700 mL 100% ethanol with 300 mL dH2O |
| Razor blade | Lord | bdk9a1ghk6 | For trichotomy |
| Sodium taurocholate | Sigma-Aldrich | 86339- 1G | CAS NUMBER- 345909-26-4 |
| Microvessel clip | Medicon Surgical | 56.87.35 | Approximator, opening 4.0 mm, closing pressure 30-40 g |
| 6-0 prolene | Bioline | 5162 | Suture line |
| Ketamin NP (cloridrato de dextrocetamina) 50mg/mL | Cristália | ||
| Xilazine 2% | Syntec | ||
| Sterile saline solution (0.9% (wt/vol) saline) | Farmace | 105851 | |
| Methyl Blue | Sigma-Aldrich Chemicals | M5528 | |
| Desmarres retractor 13 mm width | ROBOZ | RS-6672 |
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Acute Biliary Pancreatitis Model: A Method to Generate Acute Biliary Pancreatitis Mouse Model via Pancreatic Duct Infusion. J. Vis. Exp. (Pending Publication), e20750, doi: (2023).