Focus Ultrasound Based Microbubble Mediated Blood-Brain Barrier Opening: A Technique to Create Localized Transient Openings in Blood-Brain Barrier of Mouse by Sonoporation

Published: April 30, 2023

Abstract

Source: Haumann, R. et al. A High-Throughput Image-Guided Stereotactic Neuronavigation and Focused Ultrasound System for Blood-Brain Barrier Opening in Rodents. J. Vis. Exp. (2020)

This video describes a method of temporarily disrupting the blood-brain barrier (BBB) by creating transient openings using microbubble-mediated focused ultrasound (FUS). These transient local BBB openings can eventually be used to deliver larger-sized drug molecules directly into the brain parenchyma.

Protocol

All procedures involving human participants have been performed in compliance with the institutional, national and international guidelines for human welfare and have been reviewed by the local institutional review board. 1. Focused Ultrasound System NOTE: The described setup is an in-house built BBB disruption system based on commercially available components and includes a 3D-printed custom-made cone and deta…

Representative Results

Figure 1: Focused ultrasound setup. (A) Schematic representation of the focused ultrasound setup. (B) Picture of the focused ultrasound setup. The system consists of a top-down mounted transducer on a 1D linear stage over a second 2D stage for automatic 3D positioning. The transducer is built in a water-filled beam-cone, closed at the bottom with an acoustically …

Divulgaciones

The authors have nothing to disclose.

Materials

1 mL luer-lock syringe Becton Dickinson 309628 Plastipak
19 G needle Terumo Agani 8AN1938R1
23 G needle Terumo Agani 8AN2316R1
3M Transpore surgical tape Science applied to life 7000032707 Or similar
Arbitrary waveform generator Siglent SDG1025, 25 mHz, 125 MSa/s
Automated stereotact In-house built Stereotact with all elements were in-house built
Bruker In-Vivo Xtreme Bruker Includes software
Buffered NaCl solution B. Braun Melsungen AG 220/12257974/110
Buprenorfine hydrochloride Indivior UK limitd 0.324 mg
Cage enrichment: paper-pulp smart home Bio services
Carbon filter Bickford NC0111395 Omnicon f/air
Ceramic spoon
Cotton swabs
D-luciferin, potassium salt Gold Biotechnology LUCK-1
Ethanol VUmc pharmacy 70%
Evans Blue Sigma Aldrich E2129
Fresenius NaCl 0.9% Fresenius Kabi Nacl 0.9 %, 1000 mL
Histoacryl Braun Surgical Histoacryl 0.5 mL
Hydrophone Precision Acoustics
Insulin syringe Becton Dickinson 324825/324826 0.5 mL and 0.3 mL
Isoflurane TEVA Pharmachemie BV 8711218013196 250 mL
Ketamine Alfasan 10 %, 10 mL
Mouse food: Teklad global 18% protein rodent diet Envigo 2918-11416M
Neoflon catheter Becton Dickinson 391349 26 GA 0.6 x 19 mm
Oscilloscope Keysight technologies Infiniivision DSOX024A
Plastic tubes Greiner bio-one 210261 50 mL
Power amplifier Electronics & Innovation Ltd 210L Model 210L
Preamplifier DC Coupler Precision Acoustics Serial number: DCPS94
Scissors Sigma Aldrich S3146-1EA Or similar
Sedazine AST Farma 2%
SonoVue microbubbles Bracco 8 µl/mL
Sterile water Fresenius Kabi 1000 mL
Syringe Various syringes can be used
Temgesic Indivior UK limitd 0.3 mg/mL
Transducer Precision Acoustics 1 mhz
Tweezers Sigma Aldrich F4142-1EA Or similar
Ultrasound gel Parker Laboratories Inc. 01-02 Aquasonic 100
Vidisic gel Bausch + Lomb 10 g

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Focus Ultrasound Based Microbubble Mediated Blood-Brain Barrier Opening: A Technique to Create Localized Transient Openings in Blood-Brain Barrier of Mouse by Sonoporation. J. Vis. Exp. (Pending Publication), e20684, doi: (2023).

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