Source: Alieva, M. and Rios, A. C. Longitudinal intravital imaging of brain tumor cell behavior in response to an invasive surgical biopsy. J. Vis. Exp. (2019)
In this video, we perform intravital imaging of pre-injected cancer cells in a mouse model via an implanted cranial window. This high-resolution time-lapse imaging technique enables the study of dynamic behavior of cancer cells in the live mouse.
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.
1. Intravital Imaging
NOTE: The time interval between the tumor cell injection and the first intravital imaging session is dependent on the type of tumor cell line used. For the experiments shown in this protocol, 1x 105 GL261 cells were injected and imaged 10 days later.
2. Time-lapse image acquisition
The authors have nothing to disclose.
Opthalmic ointment | Kela Veterinaria | Duodrops veter kela 10 m | |
Hypnorm | VetaPharma Ltd | Hypnorm (Fentanyl citrate 0.315 mg/mL + Fluanison 10 mg/mL) |