Source: Ramirez-Moya, J. et al. In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice. J. Vis. Exp. (2019)
This video demonstrates the in vivo delivery of miRNA inhibitors as therapeutic agents against tumor growth in orthotopic mouse models. The intratumoral administration of synthetic miRNA inhibitors or antagomiRs allows them to bind and inhibit endogenous miRNAs, thereby causing mRNA upregulation and suppression of tumor growth.
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.
1. Flank Inoculation of Cells and Intratumoral antagomiR Treatment
The authors have nothing to disclose.
Basement Membrane Matrix: Matrigel Basement Membrane Matrix High Concentration | Corning | #354248 | |
AntagomiR: mirVana miRNA inhibitor | Thermo Fisher | 4464088 | In vivo ready |
In vivo delivery reagent: Invivofectamine 3.0 Reagent | Thermo Fisher | IVF 3005 | |
Negative control: mirVana miRNA Inhibitor, Negative Control #1 | Thermo Fisher | 4464077 | In vivo ready |
XenoLight D-Luciferin – K+ Salt Bioluminescent Substrate | PerkinElmer | 122799 | Diluted in PBS |
In vivo imaging software: IVISLumina II Imaging System | Caliper Life Sciences |