Murine Vertical Sleeve Gastrectomy: A Surgical Procedure to Downsize Stomach in Experimental Mouse Models

Published: April 30, 2023

Abstract

Source: Garibay, D., Cummings, B. P. A Murine Model of Vertical Sleeve Gastrectomy. J. Vis. Exp. (2017)

In this video, a vertical sleeve gastrectomy procedure is performed on an experimental mouse model. This procedure helps remove a significant portion of the stomach along its greater curvature.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Vertical Sleeve Gastrectomy and Sham Procedures On the day of surgery, weigh mice to obtain a baseline weight measurement. Equip the surgical room with an anesthesia system, heated water bath, and heating pad. Set the water bath to 37 °C and use it to warm 0.9% saline solution for irrigation. Clean the su…

Divulgaciones

The authors have nothing to disclose.

Materials

6-0 Suture Ethicon Z432 Monofilamentabsorbable/taper
7-0 Suture  Covidien 8866127-01 Monofilamentabsorbable/taper
Cotton swabs Fisherbrand 23-400-118 Small
Cotton swabs Fisherbrand 233-400-101 Large
Foil Various
Surgery drape Various
0.9% Saline solution Various
Microneedle driver Fine Science Tools 12075-14
Spring scissors Fine Science Tools 15396-00
Alcohol Various
Eye Ointment Paralube®Vet Ointment 17033-211-38
Meloxicam (Metacam) Boehringer Ingelheim 141-213 5 mg/ml
Thin tipped hemostats Fine Science Tools 13021-12
20 mL syringe Various
Enrofloxacin Baytril 08713254-186599 22.7 mg/ml

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Murine Vertical Sleeve Gastrectomy: A Surgical Procedure to Downsize Stomach in Experimental Mouse Models. J. Vis. Exp. (Pending Publication), e20421, doi: (2023).

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