Murine Vertical Sleeve Gastrectomy: A Surgical Procedure to Downsize Stomach in Experimental Mouse Models
Published: April 30, 2023
Abstract
Source: Garibay, D., Cummings, B. P. A Murine Model of Vertical Sleeve Gastrectomy. J. Vis. Exp. (2017)
In this video, a vertical sleeve gastrectomy procedure is performed on an experimental mouse model. This procedure helps remove a significant portion of the stomach along its greater curvature.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Vertical Sleeve Gastrectomy and Sham Procedures On the day of surgery, weigh mice to obtain a baseline weight measurement. Equip the surgical room with an anesthesia system, heated water bath, and heating pad. Set the water bath to 37 °C and use it to warm 0.9% saline solution for irrigation. Clean the su…
Divulgaciones
The authors have nothing to disclose.
Materials
| 6-0 Suture | Ethicon | Z432 | Monofilamentabsorbable/taper |
| 7-0 Suture | Covidien | 8866127-01 | Monofilamentabsorbable/taper |
| Cotton swabs | Fisherbrand | 23-400-118 | Small |
| Cotton swabs | Fisherbrand | 233-400-101 | Large |
| Foil | Various | ||
| Surgery drape | Various | ||
| 0.9% Saline solution | Various | ||
| Microneedle driver | Fine Science Tools | 12075-14 | |
| Spring scissors | Fine Science Tools | 15396-00 | |
| Alcohol | Various | ||
| Eye Ointment | Paralube®Vet Ointment | 17033-211-38 | |
| Meloxicam (Metacam) | Boehringer Ingelheim | 141-213 | 5 mg/ml |
| Thin tipped hemostats | Fine Science Tools | 13021-12 | |
| 20 mL syringe | Various | ||
| Enrofloxacin | Baytril | 08713254-186599 | 22.7 mg/ml |
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Murine Vertical Sleeve Gastrectomy: A Surgical Procedure to Downsize Stomach in Experimental Mouse Models. J. Vis. Exp. (Pending Publication), e20421, doi: (2023).