Functionalized Nanoparticle Targeting of Ovarian Cancer: A Technique to Facilitate Folic Acid-conjugated Nanoparticle Contrast Agent Uptake by Ovarian Cancer Cells
Published: April 30, 2023
Abstract
Source: Lusk, J. F. et al. Ovarian Cancer Detection Using Photoacoustic Flow Cytometry. J. Vis. Exp. (2020)
In this video, we demonstrate a technique to facilitate the internalization of folic acid-capped copper sulfide nanoparticles (CuS NPs). Folic acid-functionalized CuS NPs have the ability to target folate-receptors which are overexpressed in many ovarian cancer cells.
Protocol
1. Nanoparticle Synthesis and Functionalization CAUTION: All synthesis should occur in a ventilated chemical fume hood. Prior to synthesis, filter approximately 300 mL of deionized (DI) water through a 0.2 µm sterile filter. Clean a 250 mL glass round bottom flask with a detergent solution and rinse with DI water. Add 0.0134 g of CuCl2 into 100 mL of DI water to create a 1 mM solution. Add 0.015 g of folic acid (FA) to the C…
Divulgaciones
The authors have nothing to disclose.
Materials
| 0.025% Trypsin With EDTA | Corning | 25-053-Cl | |
| Amicon Ultra-15 Centrifugal Filters | Millipore | UFC903024 | |
| Amicon Ultra-4 Centrifugal Filters | Millipore | UFC803024 | |
| Bright-Line Hematocytometer | Hausser Scientific | 1492 | |
| Copper(II) Chloride | ACROS ORGANICS | 206532500 | |
| CPX Series Digital Ultrasonic Cleaning Bath | Fisherbrand | Model CPX3800 | |
| Norm-Ject 10 mL Syringes | HENKE SASS WOLF | 4100-X00V0 | |
| FBS | Sigma-Aldrich | F2442-500ML | |
| Folic Acid | Sigma-Aldrich | F7876-10G | |
| PBS | Alfa Aesar | J62036 | |
| Penicillin Streptomycin | GIBCO | 15140-122 | |
| RPMI Midum 1640 (1x) Folic Acid Free | Gibco | 27016-021 | |
| SKOV-3 Cells | ATCC | HTB-77 | |
| Sodium Hydroxide Beads | BDH | BDH9292-500G | |
| Sodium Sulfide Nonahydrate | Sigma-Aldrich | 431648-50G | |
| Trypan Blue Solution 0.4% | Amresco | K940-100ML | |
| 0.2 µm sterile syringe filter | VWR | 28145-477 | |
| Alconox | Sigma-Aldrich | 242985-1.8KG | Detergent used for cleaning glassware. |
| Tween 20 | Sigma-Aldrich | P7949-100ML |
Tags
Citar este artículo
Functionalized Nanoparticle Targeting of Ovarian Cancer: A Technique to Facilitate Folic Acid-conjugated Nanoparticle Contrast Agent Uptake by Ovarian Cancer Cells. J. Vis. Exp. (Pending Publication), e20368, doi: (2023).