Source: Lusk, J. F. et al. Ovarian Cancer Detection Using Photoacoustic Flow Cytometry. J. Vis. Exp. (2020)
In this video, we demonstrate a technique to facilitate the internalization of folic acid-capped copper sulfide nanoparticles (CuS NPs). Folic acid-functionalized CuS NPs have the ability to target folate-receptors which are overexpressed in many ovarian cancer cells.
1. Nanoparticle Synthesis and Functionalization
CAUTION: All synthesis should occur in a ventilated chemical fume hood.
2. FA-CuS NPs Uptake by Ovarian Cancer Cells
The authors have nothing to disclose.
0.025% Trypsin With EDTA | Corning | 25-053-Cl | |
Amicon Ultra-15 Centrifugal Filters | Millipore | UFC903024 | |
Amicon Ultra-4 Centrifugal Filters | Millipore | UFC803024 | |
Bright-Line Hematocytometer | Hausser Scientific | 1492 | |
Copper(II) Chloride | ACROS ORGANICS | 206532500 | |
CPX Series Digital Ultrasonic Cleaning Bath | Fisherbrand | Model CPX3800 | |
Norm-Ject 10 mL Syringes | HENKE SASS WOLF | 4100-X00V0 | |
FBS | Sigma-Aldrich | F2442-500ML | |
Folic Acid | Sigma-Aldrich | F7876-10G | |
PBS | Alfa Aesar | J62036 | |
Penicillin Streptomycin | GIBCO | 15140-122 | |
RPMI Midum 1640 (1x) Folic Acid Free | Gibco | 27016-021 | |
SKOV-3 Cells | ATCC | HTB-77 | |
Sodium Hydroxide Beads | BDH | BDH9292-500G | |
Sodium Sulfide Nonahydrate | Sigma-Aldrich | 431648-50G | |
Trypan Blue Solution 0.4% | Amresco | K940-100ML | |
0.2 µm sterile syringe filter | VWR | 28145-477 | |
Alconox | Sigma-Aldrich | 242985-1.8KG | Detergent used for cleaning glassware. |
Tween 20 | Sigma-Aldrich | P7949-100ML |