Murine Ileal Resection Model: Studying Ileectomy-Induced Bile Metabolism in Murine Model
Published: April 30, 2023
Abstract
Source: Zhang, R., et al. Ileectomy-induced Bile Overaccumulation in Mouse Intestine. J. Vis. Exp. (2017)
This video describes the detailed protocol for intestinal resection in a murine model. This technique provides insights into ileectomy-induced bile malabsorption, overaccumulation, and toxicity in the mouse intestine.
Protocol
1. Ileectomy and Anastomosis Anesthetize mice with isoflurane (2 – 3%) in a small animal incubation chamber. Determine adequate anesthetization using the toe pinch technique while the animal is on isoflurane. Remove abdominal hair by applying hair removal products and wipe hair away using surgical sponges while maintaining anesthesia. Place the mouse on a temperature-controlled small animal surgical table (Figure 1A) to maintain body temperature at 37 °C. Maint…
Representative Results
Figure 1: Surgery Procedures of Ileectomy. (A) Maintain body temperature using a temperature-controlled small animal surgical table. (B) Cover the surgery area with sterile gauzes after abdominal skin preparation. (C) Expose the small intestine through a midline abdominal incision. (D) Cecum (highlighted by the white dashed line) is marked to locate the ileum. Expose the ileum (yellow…
Divulgaciones
The authors have nothing to disclose.
Materials
| C57BL/6J | The Jackson Laboratory | ||
| Dissection microscope | Olympus | SZ61 | For surgery |
| Animal temperature controller | Physitemp Instruments, Inc | TCAT-2LV | For body temperature control |
| Isoflurane anesthetic vaporizer | VetEquip | 911104 | For anaesthesia |
| Dissection forceps | Fine Science Tools, Inc. | 11274-20 | For surgery |
| Scissors | Fine Science Tools, Inc | 14084-08 | For surgery |
| Needle holder | Roboz Surgical Instrument Co. | RS-7882 | For surgery |
| Micro knives-needle blade | Fisher Scientific | 10318-14 | For surgery |
| 6-0 monofilament suture | Ethicon | 1698G | For abdominal skin closure |
| 7-0 silk suture | Ethicon | 766G | For ligation |
| 8-0 monofilament suture | Ethicon | 1714G | For anastomosis |
| Surgical sponges | Dynarex Corp. | 3333 | For surgery |
| Small cotton-tipped applicators | Fisher Scientific | 23-400-118 | For surgery |
| Isoflurane | Piramal Healthcare Limited | 66794-013-25 | For anaesthesia |
| Buprenorphine hydrochloride | Reckitt-Benckiser Pharmaceuticals | 12496-0757-1 | For analgesia |
| 0.9% sodium chloride Injection | B. Braun Medical Inc. | 0264-7800-10 | For washing/injection |
| Povidone iodine prep solution | Dynarex Corp. | 1413 | For skin preparation |
| Puralube vet ointment | Dechra Veterinary Products | 17033-211-38 | For eye protection |
| Hair remover lotion | Church & Dwight Co., Inc. | For skin preparation | |
| Intensive care unit | ThermoCare | FW-1 | For post-surgery recovery |
| Total bile acid assay kit | Genzyme Diagnostic | DZ042A-K01 | For bile acid assay |
Citar este artículo
Murine Ileal Resection Model: Studying Ileectomy-Induced Bile Metabolism in Murine Model. J. Vis. Exp. (Pending Publication), e20337, doi: (2023).