DCE-MRI of Orthotopic Pancreatic Tumor Xenografts: A Method to Assess Microvasculature in a Target Tumor Tissue
Published: April 30, 2023
Abstract
Source: Kim, H. et al. Dynamic Contrast Enhanced Magnetic Resonance Imaging of an Orthotopic Pancreatic Cancer Mouse Model. J. Vis. Exp. (2015).
This video describes the technique of DCE-MRI for orthotropic pancreatic tumor xenografts in mice. DCE-MRI is a non-invasive method to analyze microvasculature in a target tissue, and useful to assess vascular response in a tumor following a novel therapy. This method will assist investigators to apply DCE-MRI for orthotopic gastrointestinal cancer mouse models.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Magnetic Resonance Imaging Apply MRI when tumor size is about 5 – 7 mm in diameter at usually 2~4 weeks after cell implantation. Use an MR scanner dedicated to small animal imaging or a clinical MR scanner equipped with a specialized coil for small animal imaging. NOTE: We used a 9.4T small animal MR scanner with…
Divulgaciones
The authors have nothing to disclose.
Materials
| 9.4T MR scanner | Bruker Biospin Corporation | BioSpec 94/20 USR | |
| Gadoteridol | Bracco Diagnostics Inc | NDC 0270-1111-03 | |
| Micro-polyethelene tube | Strategic Applications, Inc | #PE-10-25 | |
| 30 G blunt tip needle | Strategic Applications, Inc | 89134-194 | |
| Monitoring and gating system | SA instruments, Inc | Model 1030 | This is an MR compatiable system to measure respiratory rating and body temperature of small animals at the same time. |
| Syringe pump | New Era Pump Systems, Inc. | NE-1600 |
Citar este artículo
DCE-MRI of Orthotopic Pancreatic Tumor Xenografts: A Method to Assess Microvasculature in a Target Tumor Tissue. J. Vis. Exp. (Pending Publication), e20321, doi: (2023).