Source: Kesarwani, M., et al. Methods for Evaluating the Role of c-Fos and Dusp1 in Oncogene Dependence. J. Vis. Exp. (2019).
This video describes the technique of isolating c-kit positive cells from the bone marrow of a mouse. In the protocol, we will isolate c-kit positive cells from a transgenic mouse to validate c-Fos and Dusp1 alone or together as drug targets in leukemia.
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.
Isolation of c-Kit+ Cells from Bone Marrow
The authors have nothing to disclose.
Biological Materials | |||
IMDM | Cellgro (corning) | 15-016-CVR | |
Recombinant Murine SCF | Prospec | CYT-275 | |
Recombinant Murine Flt3-Ligand | Prospec | CYT-340 | |
Recombinant Murine IL-6 | Prospec | CYT-350 | |
Recombinant Murine IL-7 | Peprotech | 217-17 | |
FBS | Atlanta biological | S11150 | |
PBS | Corning | 21040CV | |
70 μm nylon cell stariner | Becton Dickinson | 352350 | |
LS Columns | Miltenyi | 130-042-401 | |
EDTA | Ambion | AM9261 | |
BSA | Sigma | A7906 | |
Mortor pestle | Coor tek | 60316 and 60317 | |
CD117 MicroBead Kit | Miltenyi | 130-091-224 | |
Magnet Stand | Miltenyi |