XTT Assay: A Colorimetric Assay to Assess Cell Viability and Mitochondrial Activity
Published: April 30, 2023
Abstract
Trendowski, M., et al. Generation and Quantitative Analysis of Pulsed Low Frequency Ultrasound to Determine the Sonic Sensitivity of Untreated and Treated Neoplastic Cells. J. Vis. Exp. (2015)
This article describes the protocol for XTT assay which is a colorimetric assay to assess cell viability and mitochondrial activity.
Protocol
1. XTT Assay to Determine Cell Viability and Mitochondrial Activity of Treated U937 and THP1 Cells Prior to the XTT assays, grow U937 and THP1 cells under the same conditions. Pretreat cells with the same concentration range of MeβCD for 30 min prior to sonication. Use the same ultrasound parameters as before except that cells are now sonicated using a range of 1-3 one sec pulses spaced one sec apart to develop a range of damage for the XTT kit to assess. NOTE: Many of these steps…
Divulgaciones
The authors have nothing to disclose.
Materials
| Iscove's Modified Dulbecco's Medium w/ NaHCO3 & 25mM Hepes |
Life Technologies | 12440079 | |
| Branson SLPe 40kHz Cell Disruptor with 3" (25mm) Cuphorn |
Branson Ultrasonics | 101-063-726 | sonication device |
| Tip One 100 μl and 1,000 μl Filter Tips | USA Scientific | 1120-1840, 1126-7810 | |
| 100 μl Micropipette | Wheaton | 851164 | |
| 1000 μl Micropipette | Wheaton | 851168 | |
| Forma Scientific Dual chamber water jacketed Incubator |
Forma Scientific | 3131 | |
| XTT Cell Proliferation Assay Kit | ATCC | 30-1011K | |
| 96-Well Microplate Reader | Cole-Palmer | EW-13055-54 | |
| U937 Human Monocytic Leukemia Cells | ATCC | CRL1593.2 | |
| THP1 Human Monocytic Leukemia Cells | ATCC | TIB-202 |
Citar este artículo
XTT Assay: A Colorimetric Assay to Assess Cell Viability and Mitochondrial Activity. J. Vis. Exp. (Pending Publication), e20257, doi: (2023).