Source: Steele, W. B., et al. Experimental Protocol for Examining Behavioral Response Profiles in Larval Fish: Application to the Neuro-stimulant Caffeine. J. Vis. Exp. (2018).
This video describes the photomotor response assay of zebrafish and fathead minnow larvae using an automated tracking software. The protocol measures the behavioral response of the larval fish following a sudden change in lighting condition.
1. Calibration of Video-tracking Parameters
2. Observation of Larval Fish Locomotor and Photomotor Behavior
48-well plates | Fisher Scientific | 08-772-52 | Larval zebrafish behavioral recording chambers |
24-well plates | VWR | 10062-896 | Larval fathead minnow behavioral recording chambers |