Calcium Imaging: A Method to Visualize Neural Activity in Live C. elegans

Published: April 30, 2023

Abstract

Source: Turek, M. et al. Agarose Microchambers for Long-term Calcium Imaging of Caenorhabditis elegans. J. Vis. Exp. (2015).

This video introduces the concepts behind calcium imaging and includes an example protocol for time-lapse imaging of worms contained in molded agarose wells.

Protocol

This protocol is an excerpt from Turek et al, Agarose Microchambers for Long-term Calcium Imaging of Caenorhabditis elegans, J. Vis. Exp. (2015). 1. Calcium Imaging Use transgenic strains expressing genetically encoded calcium sensors such as HBR16 (goeIs5[pnmr-1::SL1-GCaMP3.35-SL2::unc-54-3'UT…

Materials

LED system CoolLED pE-2
Compound microscope Nikon TiE
EMCCD camera Andor iXon 897 now sold as iXon Ultra 897
Z-stage for the microscope Prior Nano Scan Z
X-Y stage for the microscope Prior Proscan III
Red light filter Chroma ET660/50m
35 mm Petri dish Falcon Falcon Disposable Petri Dishes, Sterile, Corning

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Citar este artículo
Calcium Imaging: A Method to Visualize Neural Activity in Live C. elegans. J. Vis. Exp. (Pending Publication), e20164, doi: (2023).

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