JOVE JOURNAL

Genética

Showing 169 - 180 in 420 videos.

High-Throughput DNA Plasmid Multiplexing and Transfection Using Acoustic Nanodispensing Technology

Béatrice Colin¹, Nicolas Rocq², Benoit Deprez¹, Cyril Couturier¹

¹Institut Pasteur de Lille, Institut National de la Santé et de la Recherche Médicale (Inserm) U1177, Drugs and Molecules for Living Systems,Université de Lille, ²L'atelier du Développeur, Marquette lez Lille

Journal (Genetics)

Introducing a Gene Knockout Directly Into the Amastigote Stage of Trypanosoma cruzi Using the CRISPR/Cas9 System

Yukie Akutsu¹, Motomichi Doi¹, Koji Furukawa¹, Yuko Takagi¹

¹Biomedical Research Institute,National Institute of Advanced Industrial Science and Technology (AIST)

Journal (Genetics)

Discrimintion and Mapping of the Primary and Processed Transcripts in Maize Mitochondrion Using a Circular RT-PCR-based Strategy

Yafeng Zhang^1,2, Xun Liao², Peihua Luo², Kui Peng², Wenyi Ye², Tengxiang Lian^1,2, Qibin Ma^1,2, Hai Nian^1,2

¹State Key Laboratory of Conservation and Utilization of Subtropical Agro-Bioresources,South China Agricultural University, ²Guangdong Provincial Key Laboratory of Plant Molecular Breeding, College of Agriculture,South China Agricultural University

Journal (Genetics)

Measurement of BK-polyomavirus Non-Coding Control Region Driven Transcriptional Activity Via Flow Cytometry

Johannes Korth^1,2, Helene Sertznig², Siegfried Moyrer², Adrian Atilla Nicolas Doevelaar^2,3, Timm Henning Westhoff³, Nina Babel³, Oliver Witzke⁴, Andreas Kribben¹, Ulf Dittmer², Marek Widera²

¹Department of Nephrology,University of Duisburg-Essen, University Hospital Essen, ²Institute for Virology,University of Duisburg-Essen, University Hospital Essen, ³Center for Translational Medicine, Medical Department I, Marien Hospital Herne,University Hospital of the Ruhr-University of Bochum, ⁴Department of Infectious Diseases, University of Duisburg-Essen,University Hospital Essen Hufelandstr

Journal (Genetics)

Characterizing Mutational Load and Clonal Composition of Human Blood

Axel Rosendahl Huber¹, Freek Manders¹, Rurika Oka¹, Ruben van Boxtel¹

¹Princess Máxima Center for Pediatric Oncology

Journal (Genetics)

In Vivo Forward Genetic Screen to Identify Novel Neuroprotective Genes in Drosophila melanogaster

Olivia Gevedon¹, Harris Bolus¹, Shu Hui Lye¹, Keaton Schmitz¹, Jesualdo Fuentes-González¹, Kathryn Hatchell², Lyndsey Bley², Jason Pienaar¹, Carin Loewen², Stanislava Chtarbanova¹

¹Department of Biological Sciences,University of Alabama, ²Department of Genetics,University of Wisconsin-Madison

Journal (Genetics)

Identification of Footprints of RNA:Protein Complexes via RNA Immunoprecipitation in Tandem Followed by Sequencing (RIPiT-Seq)

Lauren Woodward¹, Pooja Gangras¹, Guramrit Singh¹

¹Department of Molecular Genetics, Center for RNA Biology,The Ohio State University

Journal (Genetics)

Antibody-Free Assay for RNA Methyltransferase Activity Analysis

Samantha B. Shelton¹, Blerta Xhemalce¹

¹Department of Molecular Biosciences,University of Texas at Austin

Journal (Genetics)

Direct Gene Knock-out of Axolotl Spinal Cord Neural Stem Cells via Electroporation of CAS9 Protein-gRNA Complexes

Wilson Pak-Kin Lou*^1,2, Liqun Wang*³, Cheng Long¹, Lei Liu³, Ji-Feng Fei³

¹School of Life Sciences,South China Normal University, ²The Research Institute of Molecular Pathology (IMP),Vienna Biocenter (VBC), ³Institute for Brain Research and Rehabilitation (IBRR),South China Normal University

Journal (Genetics)

Transcription Start Site Mapping Using Super-low Input Carrier-CAGE

Nevena Cvetesic^1,2, Elena Pahita^1,2, Boris Lenhard^1,2,3

¹Institute of Clinical Sciences, Faculty of Medicine,Imperial College London, ²MRC London Institute of Medical Sciences, ³Sars International Centre for Marine Molecular Biology,University of Bergen

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JOVE JOURNAL

Genética

High-Throughput DNA Plasmid Multiplexing and Transfection Using Acoustic Nanodispensing Technology

Introducing a Gene Knockout Directly Into the Amastigote Stage of Trypanosoma cruzi Using the CRISPR/Cas9 System

Discrimintion and Mapping of the Primary and Processed Transcripts in Maize Mitochondrion Using a Circular RT-PCR-based Strategy

Single-cell RNA Sequencing and Analysis of Human Pancreatic Islets

In Vitro Biochemical Assays using Biotin Labels to Study Protein-Nucleic Acid Interactions

Measurement of BK-polyomavirus Non-Coding Control Region Driven Transcriptional Activity Via Flow Cytometry

Characterizing Mutational Load and Clonal Composition of Human Blood

In Vivo Forward Genetic Screen to Identify Novel Neuroprotective Genes in Drosophila melanogaster

Identification of Footprints of RNA:Protein Complexes via RNA Immunoprecipitation in Tandem Followed by Sequencing (RIPiT-Seq)

Antibody-Free Assay for RNA Methyltransferase Activity Analysis

Direct Gene Knock-out of Axolotl Spinal Cord Neural Stem Cells via Electroporation of CAS9 Protein-gRNA Complexes

Transcription Start Site Mapping Using Super-low Input Carrier-CAGE