JOVE JOURNAL

Biología

Showing 1,249 - 1,260 in 3,287 videos.

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Jo Anne Stratton*^1,2,3, Sarthak Sinha*², Wisoo Shin², Elodie Labit², Tak-Ho Chu^1,4, Prajay T. Shah², Rajiv Midha^1,4, Jeff Biernaskie^1,2,3

¹Hotchkiss Brain Institute,University of Calgary, ²Department of Comparative Biology and Experimental Medicine, Faculty of Veterinary Medicine,University of Calgary, ³Alberta Children's Hospital Research Institute,University of Calgary, ⁴Department of Clinical Neurosciences, Cumming School of Medicine,University of Calgary

Journal (Biology)

Isolation of CD4⁺ T-cells and Analysis of Circulating T-follicular Helper (cTfh) Cell Subsets from Peripheral Blood Using 6-color Flow Cytometry

Elliot Byford¹, Matthew Carr¹, Lucia Piñon², Matthew J. Ahearne¹, Simon D. Wagner¹

¹Leicester Cancer Research Centre and Ernest and Helen Scott Haematology Research Institute,University of Leicester, ²MRC Toxicology Unit,University of Leicester

Journal (Biology)

Preparation of Prokaryotic and Eukaryotic Organisms Using Chemical Drying for Morphological Analysis in Scanning Electron Microscopy (SEM)

Madison A. Koon¹, Khadijah Almohammed Ali¹, Robert M. Speaker¹, James P. McGrath¹, Eric W. Linton¹, Michelle L. Steinhilb¹

¹Department of Biology,Central Michigan University

Journal (Biology)

Myeloid Innate Signaling Pathway Regulation by MALT1 Paracaspase Activity

Adeline Unterreiner*¹, Ratiba Touil*¹, Daniele Anastasi¹, Nicolas Dubois¹, Satoru Niwa¹, Thomas Calzascia¹, Frederic Bornancin¹

¹Autoimmunity, Transplantation and Inflammation,Novartis Institutes for BioMedical Research

Journal (Biology)

Measurement of Energy Metabolism in Explanted Retinal Tissue Using Extracellular Flux Analysis

Jeffrey R. Millman^1,2, Teresa Doggett³, Christina Thebeau³, Sheng Zhang³, Clay F. Semenkovich¹, Rithwick Rajagopal³

¹Division of Metabolism, Endocrinology and Lipid Research, Department of Medicine,Washington University School of Medicine, ²Department of Biomedical Engineering,Washington University in Saint Louis, ³Department of Ophthalmology and Visual Science,Washington University School of Medicine

Journal (Biology)

Isolation, Characterization, and Differentiation of Cardiac Stem Cells from the Adult Mouse Heart

Santosh K. Yadav¹, Paras K. Mishra^1,2

¹Department of Cellular and Integrative Physiology,University of Nebraska Medical Center, ²Department of Anesthesiology,University of Nebraska Medical Center

Journal (Biology)

A BW Reporter System for Studying Receptor-Ligand Interactions

Shlomo Elias^1,2, Shira Kahlon², Alexandra Duev-Cohen², Ofer Mandelboim²

¹Department of Hematology,Hadassah - Hebrew University Medical Center, ²The Lautenberg Center for General and Tumor Immunology, Department of Immunology and Cancer Research, Institute for Medical Research Israel Canada (IMRIC),Hadassah - Hebrew University Medical Center

Journal (Biology)

Quantifying Leukocyte Egress via Lymphatic Vessels from Murine Skin and Tumors

Maria M. Steele¹, Madeline J. Churchill², Alec P. Breazeale¹, Ryan S. Lane¹, Nicholas A. Nelson¹, Amanda W. Lund^1,2,3,4

¹Department of Cell, Developmental, & Cancer Biology,Oregon Health and Science University, ²Department of Molecular Microbiology & Immunology,Oregon Health and Science University, ³Department of Dermatology,Oregon Health and Science University, ⁴Knight Cancer Institute,Oregon Health and Science University

Journal (Biology)

Applications of Spatio-temporal Mapping and Particle Analysis Techniques to Quantify Intracellular Ca²⁺ Signaling In Situ

Bernard T. Drumm¹, Grant W. Hennig², Salah A. Baker¹, Kenton M. Sanders¹

¹Department of Physiology and Cell Biology,University of Nevada Reno School of Medicine, ²Department of Pharmacology, The Robert Larner, M.D. College of Medicine,University of Vermont

Journal (Biology)

The MUB₄₀ Peptide for Use in Detecting Neutrophil-Mediated Inflammation Events

Mark C. Anderson^1,2, Louise Injarabian^1,2,3, Antonin Andre^1,2,3, Regis Tournebize^1,2,4, Benoit S. Marteyn^1,2

¹Unité de Pathogénie Microbienne Moléculaire,Institut Pasteur, ²INSERM Unité 1202,Institut Pasteur, ³Institut de Biochimie et Génétique Cellulaires,Université Bordeaux Segalen, ⁴Unit of Technology and Service Photonic BioImaging,Institut Pasteur

Journal (Biology)

A Method for Islet Transplantation to the Omentum in Mouse

Ying Lu*¹, Shangyou Zou*¹, Suzanne Bertera*², Rita Bottino², David K.C. Cooper³, Zhengzhao Liu¹, Yi Huang¹, Chengjun Wang¹, Chungu Hong¹, Tian He¹, Hancheng Zhang¹, Qin Huo⁴, Hongxing Fu⁵, Zhiming Cai¹, Lisha Mou¹

¹Shenzhen Xenotransplantation Medical Engineering Research and Development Center, Institute of Translational Medicine, Shenzhen Second People's Hospital,First Affiliated Hospital of Shenzhen University, Shenzhen University School of Medicine, Shenzhen University Health Science Center, ²Institute for Cellular Therapeutics,Allegheny-Singer Research Institute, ³Xenotransplantation Program/Department of Surgery,The University of Alabama at Birmingham, ⁴College of Life Science and Oceanography,Shenzhen University, ⁵School of Pharmcy,Wenzhou Medical College

Journal (Biology)

Quantitative Immunoblotting of Cell Lines as a Standard to Validate Immunofluorescence for Quantifying Biomarker Proteins in Routine Tissue Samples

Alison M. Moore^1,2, Lee R. Boudreau³, Shakeel Virk³, David P. LeBrun^1,2

¹Department of Pathology and Molecular Medicine,Queen's University, ²Division of Cancer Biology and Genetics,Queen's Cancer Research Institute, ³Queen's Laboratory for Molecular Pathology, Department of Pathology and Molecular Medicine,Queen's University

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