Inmunología y contagio

Calcium Imaging of Virus-Infected Human Intestinal Organoid Monolayers

Calcium Imaging of Virus-Infected Human Intestinal Organoid Monolayers

January 19, 2024

Author Spotlight: Investigating Viral Disruption of Intestinal Epithelial Signaling – Research Insights and Future Directions

Preparation of Human Intestinal Organoid Monolayers for Live Fluorescence Imaging

Viral Infection of Human Intestinal Organoid Monolayers for Live Fluorescence Imaging

Live Imaging of Virus-Infected Human Intestinal Organoid Monolayers

J. Thomas Gebert¹, Francesca J. Scribano¹, Kristen A. Engevik¹, Joseph M. Hyser¹

¹Alkek Center for Metagenomics and Microbiome Research, Department of Molecular Virology and Microbiology,Baylor College of Medicine

Summary

This protocol describes an approach for performing calcium imaging in virus-infected human intestinal organoids and offers an approach to analysis.

Article