Calcium Imaging of Virus-Infected Human Intestinal Organoid Monolayers

January 19, 2024

Author Spotlight: Investigating Viral Disruption of Intestinal Epithelial Signaling – Research Insights and Future Directions

Preparation of Human Intestinal Organoid Monolayers for Live Fluorescence Imaging

Viral Infection of Human Intestinal Organoid Monolayers for Live Fluorescence Imaging

Live Imaging of Virus-Infected Human Intestinal Organoid Monolayers

J. Thomas Gebert, Francesca J. Scribano, Kristen A. Engevik, Joseph M. Hyser

¹Alkek Center for Metagenomics and Microbiome Research, Department of Molecular Virology and Microbiology,Baylor College of Medicine

Summary

This protocol describes an approach for performing calcium imaging in virus-infected human intestinal organoids and offers an approach to analysis.

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