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Sigma’s Non-specific Protease Activity Assay – Casein as a Substrate
JoVE Journal
Biologie
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JoVE Journal Biologie
Sigma’s Non-specific Protease Activity Assay – Casein as a Substrate

Sigma’s Non-specific Protease Activity Assay – Casein as a Substrate

DOI:
10.3791/899-v

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11:37 min

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September 17, 2008

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1Sigma Aldrich

Kapitel

  • 00:00Titel
  • 01:23Introduction
  • 01:52Preparation of Reagents
  • 04:33Setting Up the Protease Assay and Standard Curves
  • 08:12Measuring Absorbance and Calculating Enzyme Activity
  • 10:59Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

Proteases break peptide bonds. In the lab, it is often necessary to measure and/or compare the activity of proteases. Sigma's non-specific protease activity assay may be used as a standardized procedure to determine the activity of proteases.

Tags

SigmaNon-specific Protease Activity AssayCasein SubstrateQuality Control ProceduresAmino Acid TyrosinePeptide FragmentsFolin And Ciocalteus PhenolFolin’s ReagentBlue Colored ChromophoreAbsorbance ValueSpectrophotometerTyrosine ReleaseProtease ActivityStandard CurveUnitsMicromoles

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