Journal
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Entwicklungsbiologie
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Protocol to Prepare Demineralized FFPE Bone Tissue Samples with RNA Integrity
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Author Spotlight: Exploring Advanced Therapeutic Targets in Osteosarcoma Through Spatial Transcriptomics
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Entwicklungsbiologie
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JoVE Journal Entwicklungsbiologie
Methods to Enable Spatial Transcriptomics of Bone Tissues

Author Spotlight: Exploring Advanced Therapeutic Targets in Osteosarcoma Through Spatial Transcriptomics

Protocol to Prepare Demineralized FFPE Bone Tissue Samples with RNA Integrity

DOI:
10.3791/66850-v

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01:13 min

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May 03, 2024

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, , ,
1Department of Periodontics and Preventive Dentistry,University of Pittsburgh School of Dental Medicine, 2Center for Craniofacial Regeneration,University of Pittsburgh School of Dental Medicine, 3UPMC Presbyterian, 4UPMC Shadyside, 5Musculoskeletal Oncology Laboratory, Department of Orthopaedic Surgery,University of Pittsburgh, 6UPMC Hillman Cancer Center, 7Departments of Anatomic Pathology and General Surgical Oncology,University of Pittsburgh, 8Department of Medicine,University of Pittsburgh School of Medicine, 9University of Pittsburgh UPMC Hillman Cancer Center, 10McGowan Institute for Regenerative Medicine,University of Pittsburgh

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Spatial TranscriptomicsRNA PreservationDecalcificationParaffin-embedded SamplesOsteosarcoma

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