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Two-Step Cell Digestion of Mouse Carotid Artery
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Vascular Tissue Dissociation and Exploring Single-Cell Subclusters for Targeted Therapy (Video) | JoVE
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Biologie
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JoVE Journal Biologie
Preparation of a Single-Cell Suspension from Mouse Carotid Arteries for Single-Cell Sequencing

Vascular Tissue Dissociation and Exploring Single-Cell Subclusters for Targeted Therapy (Video) | JoVE

Two-Step Cell Digestion of Mouse Carotid Artery

DOI:
10.3791/65863-v

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01:16 min

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January 19, 2024

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, , , ,
1Cyrus Tang Hematology Center, Cyrus Tang Medical Institute,Soochow University, 2Collaborative Innovation Center of Hematology of Jiangsu Province,Soochow University, 3Suzhou Key Laboratory of Thrombosis and Vascular Biology,Soochow University

Tags

Keywords: Single-cell SuspensionMouse Carotid ArteriesSingle-cell SequencingCarotid StenosisCarotid AtherosclerosisTwo-step Digestion MethodCollagenase/DNaseTrypsinCell ViabilityCell ConcentrationVascular Smooth Muscle Cells (VSMCs)FibroblastsEndothelial Cells (ECs)Macrophages And Dendritic Cells (M&DCs)

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Vascular Tissue Dissociation and Exploring Single-Cell Subclusters for Targeted Therapy (Video) | JoVE
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