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Histone Proteomic Analysis and Post-Translational Modification Characterization
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Author Spotlight: Enhanced Histone PTM Isomer Identification Through LC-TIMS-ToF MS/MS and PASEF
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Chemie
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JoVE Journal Chemie
Histone Modification Screening using Liquid Chromatography, Trapped Ion Mobility Spectrometry, and Time-Of-Flight Mass Spectrometry

Author Spotlight: Enhanced Histone PTM Isomer Identification Through LC-TIMS-ToF MS/MS and PASEF

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01:32 min

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January 12, 2024

DOI:

10.3791/65589-v

DOI:
10.3791/65589-v

•

01:32 min
January 12, 2024

•

12 Views
, , , , , , ,
1Department of Chemistry and Biochemistry,Florida International University, 2Bruker Daltonics Inc., 3Department of Biochemistry and Molecular Biophysics,Washington University School of Medicine, 4Biomolecular Sciences Institute,Florida International University

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Histone ModificationLiquid ChromatographyTrapped Ion Mobility SpectrometryTime-of-flight Mass SpectrometryPost-translational ModificationsEpigeneticsProteomicsBioinformatics

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Fernandez-Rojas, M., Fuller, C. N., Valadares Tose, L., Willetts, M., Park, M. A., Bhanu, N. V., Garcia, B. A., Fernandez-Lima, F. Histone Modification Screening using Liquid Chromatography, Trapped Ion Mobility Spectrometry, and Time-Of-Flight Mass Spectrometry. J. Vis. Exp. (203), e65589, doi:10.3791/65589 (2024).

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