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Quantification of Viral Vectors in Tears Using ddPCR for Bioshedding Studies
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Author Spotlight: Addressing Regulatory Gaps in Molecular Studies by Quantifying Viral Vectors in Complex Matrices
JoVE Journal
Biologie
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JoVE Journal Biologie
A Validatable Droplet Digital Polymerase Chain Reaction Assay for the Detection of Adeno-Associated Viral Vectors in Bioshedding Studies of Tears

Author Spotlight: Addressing Regulatory Gaps in Molecular Studies by Quantifying Viral Vectors in Complex Matrices

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02:04 min

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July 14, 2023

DOI:

10.3791/65495-v

DOI:
10.3791/65495-v

•

02:04 min
July 14, 2023

•

3 Views
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1Cell and Gene Therapy,KCAS Bioanalytical and Biomarker Services

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Adeno-associated Viral VectorsBiosheddingDroplet Digital PCRQuantificationTearsValidationRegulatoryMolecular Assay

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Longacre, B., Souaysene, A., Vyhlidal, C. A., Pennington, M. R. A Validatable Droplet Digital Polymerase Chain Reaction Assay for the Detection of Adeno-Associated Viral Vectors in Bioshedding Studies of Tears. J. Vis. Exp. (197), e65495, doi:10.3791/65495 (2023).

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