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Biochemistry

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JoVE Journal Biochemistry

Analysis of Fluorescent-Stained Lipid Droplets with 3D Reconstruction for Hepatic Steatosis Assessment (Video) | JoVE

Analysis of Fluorescent-Stained Lipid Droplets with 3D Reconstruction for Hepatic Steatosis Assessment (Video) | JoVE

Assessing Hepatic Steatosis with 3D Fluorescence Lipid Droplet Reconstruction

Article DOI: 10.3791/65206-v • 01:25 min • June 2nd, 2023

June 2nd, 2023 •

Karina Garcia^1,2,3,4, André Alves^1,2,3, Teresa M. Ribeiro-Rodrigues^1,2,3, Flávio Reis^1,2,3, Sofia Viana^1,2,3,4

¹Institute of Pharmacology & Experimental Therapeutics & Coimbra Institute for Clinical and Biomedical Research (iCBR), Faculty of Medicine, University of Coimbra, ²Center for Innovative Biomedicine and Biotechnology (CIBB), University of Coimbra, ³Clinical Academic Center of Coimbra (CACC), ⁴Polytechnic Institute of Coimbra, Coimbra Health School

Tags

Fluorescent-stained Lipid Droplets 3D Reconstruction Hepatic Steatosis Assessment Histological Staining Microvesicular Steatosis Oil Red Pro-BODIPY Hydrophobicity Lipid Droplet Tagging Environment Polarity Immunochemistry Techniques Subcellular Antigens Lipid Droplet Biogenesis Lipid Droplet Dynamics Microvesicular Hepatic Steatosis

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