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Antistoffopptaksanalyse for sporing av hakk/delta-endocytose under asymmetrisk deling av sebrafisk radiale glia-stamfedre
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Entwicklungsbiologie
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JoVE Journal Entwicklungsbiologie
Antibody Uptake Assay for Tracking Notch/Delta Endocytosis During the Asymmetric Division of Zebrafish Radial Glia Progenitors

Antistoffopptaksanalyse for sporing av hakk/delta-endocytose under asymmetrisk deling av sebrafisk radiale glia-stamfedre

1,778 Views

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09:06 min

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January 20, 2023

DOI:

10.3791/65030-v

DOI:
10.3791/65030-v

•

09:06 min
January 20, 2023

•

14 Views
,
1Chan Zuckerberg Biohub, 2Department of Bioengineering and Therapeutic Sciences,University of California San Francisco, 3Programs in Human Genetics and Biological Sciences,University of California San Francisco

Tags

Antibody Uptake AssayNotch/Delta EndocytosisAsymmetric DivisionZebrafish Radial Glia ProgenitorsNotch SignalingSulfate DecisionPatterningDevelopmentMetazoansAnti-Dld AntibodyAnti-mouse IgG Atto-647N AntibodyBrain VentriclesEmbryonic DevelopmentMicroinjectionFluorescence Microscopy

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Zhao, X., Guo, S. Antibody Uptake Assay for Tracking Notch/Delta Endocytosis During the Asymmetric Division of Zebrafish Radial Glia Progenitors. J. Vis. Exp. (191), e65030, doi:10.3791/65030 (2023).

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