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Culture of Piglet Intestinal 3D Organoids from Cryopreserved Epithelial Crypts and Establishment of Cell Monolayers
JoVE Journal
Biologie
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JoVE Journal Biologie
Culture of Piglet Intestinal 3D Organoids from Cryopreserved Epithelial Crypts and Establishment of Cell Monolayers

Culture of Piglet Intestinal 3D Organoids from Cryopreserved Epithelial Crypts and Establishment of Cell Monolayers

1,576 Views

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08:19 min

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February 10, 2023

DOI:

10.3791/64917-v

DOI:
10.3791/64917-v

•

08:19 min
February 10, 2023

•

6 Views
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1GenPhySE,Université de Toulouse, INRAE, ENVT, 2Lallemand Animal Nutrition, 3Institut NuMeCan, INRAE, INSERM,Université de Rennes, 4FG 16: Mycotic and Parasitic Agents and Mycobacteria,Robert Koch-Institute

Tags

Pig Intestinal EpitheliumIntestinal Organoids3D OrganoidsCryopreserved CryptsEpithelial Crypts2D MonolayersNutrient TransportBarrier FunctionHost-microbe InteractionsJejunumDuodenumIleumColonExtracellular MatrixECMCulture MediumPassagingDissociationDMEMRock Inhibitor

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Mussard, E., Lencina, C., Boudry, G., Achard, C. S., Klotz, C., Combes, S., Beaumont, M. Culture of Piglet Intestinal 3D Organoids from Cryopreserved Epithelial Crypts and Establishment of Cell Monolayers. J. Vis. Exp. (192), e64917, doi:10.3791/64917 (2023).

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