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Genbewerking van primaire resusapen B-cellen
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09:53 min
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February 10, 2023
DOI:
1Laboratory of Molecular Immunology,The Rockefeller University, 2Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases,National Institutes of Health, 3Laboratory of Retrovirology,The Rockefeller University, 4Howard Hughes Medical Institute,The Rockefeller University, 5Laboratory of Applied Virology and Precision Medicine,King Abdullah University of Science and Technology (KAUST), 6Vaccine and Immunotherapy Center,Wistar Institute
Summary
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We presenteren een methode voor het kweken en bewerken van primaire resusapen B-cellen met behulp van CRISPR / Cas9 en recombinant adeno-geassocieerd virus serotype 6 voor de studie van B-celtherapieën.
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Cite this Article
Hartweger, H., Gautam, R., Nishimura, Y., Schmidt, F., Yao, K., Escolano, A., Jankovic, M., Martin, M. A., Nussenzweig, M. C. Gene Editing of Primary Rhesus Macaque B Cells. J. Vis. Exp. (192), e64858, doi:10.3791/64858 (2023).
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