Journal
/
Biochemie
/
High-Throughput Protein Crystallization via Microdialysis
JoVE Journal
Biochemie
This content is Free Access.
JoVE Journal Biochemie
High-Throughput Protein Crystallization via Microdialysis

High-Throughput Protein Crystallization via Microdialysis

DOI:
10.3791/64744-v

•

06:18 min

•

March 03, 2023

•

, , , ,
1National Physical Laboratory, 2SWISSCI LTD

Kapitel

  • 00:04Introduction
  • 00:44Setting up the Microdialysis Plate
  • 02:37Large-Scale Crystallization Using Dialyzer Tubes
  • 03:52Results: Protein Microcrystal Formation in Microdialysis Plates and Dialyzer Tubes
  • 05:46Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

The presented protocol describes a straightforward approach for screening protein crystallization conditions and crystal growth using a 96-well high-throughput dialysis plate. The use of dialyzer tubes for the large-scale growth of microcrystals is also demonstrated for serial crystallography and MicroED applications.

Tags

Keywords: Protein CrystallizationMicrodialysisHigh-throughputDialysis96-well PlateCrystal GrowthLow-volume PipettingMultichannel PipetteUV Cover FilmDialysis SolutionTemperature-controlled IncubatorLarge-scale Crystallization

Article

Embed

ZUR WIEDERGABELISTE HINZUFÜGEN

Usage Statistics

Verwandte Videos

check_url/de/64744?article_type=v

Method for Identifying Small Molecule Inhibitors of the Protein-protein Interaction Between HCN1 and TRIP8b
check_url/de/64744?article_type=v

Production, Crystallization and Structure Determination of C. difficile PPEP-1 via Microseeding and Zinc-SAD
check_url/de/64744?article_type=v

A Protein Preparation Method for the High-throughput Identification of Proteins Interacting with a Nuclear Cofactor Using LC-MS/MS Analysis
check_url/de/64744?article_type=v

Recombinant Protein Expression, Crystallization, and Biophysical Studies of a Bacillus-conserved Nucleotide Pyrophosphorylase, BcMazG
check_url/de/64744?article_type=v

High Throughput, Absolute Determination of the Content of a Selected Protein at Tissue Levels Using Quantitative Dot Blot Analysis (QDB)
check_url/de/64744?article_type=v

Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions
check_url/de/64744?article_type=v

Strategic Screening and Characterization of the Visual GPCR-mini-G Protein Signaling Complex for Successful Crystallization
check_url/de/64744?article_type=v

An Economical and Versatile High-Throughput Protein Purification System Using a Multi-Column Plate Adapter
check_url/de/64744?article_type=v

A “Dual-Addition” Calcium Fluorescence Assay for the High-Throughput Screening of Recombinant G Protein-Coupled Receptors
check_url/de/64744?article_type=v

High-Throughput Screening to Obtain Crystal Hits for Protein Crystallography (Video) | JoVE

Read Article