Differentiation of Functional Osteoclasts from Human Peripheral Blood CD14<sup>+</sup> Monocytes

Patricia Riedlova; Shatakshi Sood; Carl S. Goodyear; Cecilia Ansalone

doi:10.3791/64698

Journal

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Immunologie und Infektion

/

Differentiation of Functional Osteoclasts from Human Peripheral Blood CD14⁺ Monocytes

JoVE Journal
Immunologie und Infektion

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JoVE Journal Immunologie und Infektion

Differentiation of Functional Osteoclasts from Human Peripheral Blood CD14⁺ Monocytes

1,943 Views

•

11:52 min

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January 27, 2023

DOI:

10.3791/64698-v

DOI:

10.3791/64698-v

•

11:52 min

January 27, 2023

•

22 Views

Patricia Riedlova*¹, Shatakshi Sood*¹, Carl S. Goodyear¹, Cecilia Ansalone¹

¹School of Infection & Immunity,University of Glasgow

Summary

Osteoclasts are key bone-resorbing cells in the body. This protocol describes a reliable method for the in vitro differentiation of osteoclasts from human peripheral blood monocytes. This method can be used as an important tool to further understand osteoclast biology in homeostasis and in diseases.

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Differentiation of Functional Osteoclasts from Human Peripheral Blood CD14+ Monocytes

Summary

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Differentiation of Functional Osteoclasts from Human Peripheral Blood CD14⁺ Monocytes