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Entwicklungsbiologie
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从健康和视网膜疾病特异性人诱导的多能干细胞中生成视网膜类器官
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Entwicklungsbiologie
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JoVE Journal
Entwicklungsbiologie
Generation of Retinal Organoids from Healthy and Retinal Disease-Specific Human-Induced Pluripotent Stem Cells
Please note that all translations are automatically generated.
Click here for the English version.
从健康和视网膜疾病特异性人诱导的多能干细胞中生成视网膜类器官
DOI:
10.3791/64509-v
•
09:47 min
•
December 09, 2022
•
Sudipta Mahato*
1,2
,
Trupti Agrawal*
1,2
,
Divya Pidishetty*
1,2
,
Savitri Maddileti
,
Vinay Kumar Pulimamidi
3
,
Indumathi Mariappan
1
Centre for Ocular Regeneration, Prof. Brien Holden Eye Research Centre
,
LV Prasad Eye Institute
,
2
Manipal Academy of Higher Education
,
3
Schepens Eye Research Institute, Massachusetts Eye and Ear, Harvard Medical School
Kapitel
00:05
Introduction
01:19
Passaging and Plating of hiPSCs to Initiate Retinal Lineage Differentiation
02:40
Differentiation of hiPSCs into Eye Fields and Retinal Lineage
04:38
Harvesting of Retinal Organoids
06:41
Results: Characterization of Retinal Organoids and RPE Cells Derived From Normal and RB1
-/-
Mutant iPSCs
09:06
Conclusion
Summary
Automatische Übersetzung
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Automatische Übersetzung
该协议描述了一种将hiPSCs分化为眼场簇并使用涉及贴壁和悬浮培养系统的简化培养条件生成神经视网膜类器官的有效方法。其他眼细胞类型,如RPE和角膜上皮,也可以在视网膜培养物中从成熟的眼视野中分离出来。
Tags
Retinal Organoids
Human Induced Pluripotent Stem Cells
Retinal Degeneration
Retinal Pigment Epithelium
In Vitro Models
Cell-based Therapies
Eye Development
Inherited Retinal Diseases
Differentiation
Adherent Culture
Suspension Culture
Retinal Cups
Spatial Positioning
Morphological Features
Cell Dissociation
IPSC Culture
Differentiation Induction Medium
BFGF
Noggin
Article
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