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A Soluble Tetrazolium-Based Reduction Assay to Evaluate the Effect of Antibodies on Candida tropicalis Biofilms
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Immunologie und Infektion
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JoVE Journal Immunologie und Infektion
A Soluble Tetrazolium-Based Reduction Assay to Evaluate the Effect of Antibodies on Candida tropicalis Biofilms

A Soluble Tetrazolium-Based Reduction Assay to Evaluate the Effect of Antibodies on Candida tropicalis Biofilms

DOI:
10.3791/64425-v

•

06:50 min

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September 16, 2022

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, ,
1Department of Biosciences and Bioengineering,Indian Institute of Technology Roorkee

Kapitel

  • 00:04Introduction
  • 01:26C. tropicalis Biofilm Formation
  • 02:40Treatment of Biofilm with Antibodies
  • 03:30Biofilm Metabolic Activity Estimation
  • 05:14Results: Effectiveness of Sap2-Specific Polyclonal Antibodies Against Preformed C. tropicalis Biofilms
  • 06:04Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

A 96-well microtiter plate-based protocol using a 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-carboxanilide-2H-tetrazolium (XTT) reduction assay is described herein, to study antibodies' effects on biofilms formed by C. tropicalis. This in vitro protocol can be used to check the effect of potential new antifungal compounds on the metabolic activity of Candida species cells in biofilms.

Tags

BiofilmCandida TropicalisAntibodyAntifungalMetabolic ActivityTetrazolium-based Reduction Assay96-well PlateRPMI 1640 MOPS MediumPBS WashSerum DilutionNo-fungus Negative Control

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