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Kompost mikrokosmos som mikrobielt forskellige, naturlignende miljøer til mikrobiomforskning i Caenorhabditis elegans
JoVE Journal
Biologie
This content is Free Access.
JoVE Journal Biologie
Compost Microcosms as Microbially Diverse, Natural-like Environments for Microbiome Research in Caenorhabditis elegans
DOI:

07:19 min

September 13, 2022

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Kapitel

  • 00:04Introduction
  • 00:36Preparation of Compost
  • 01:20Preparation of Compost Microcosms and Raising Worms in Compost Microcosms
  • 02:39Preparing a Baermann Funnel, Harvesting Worms from Microcosms, and Collecting the Respective Soil Samples
  • 04:17Washing and Surface Sterilization of the Harvested Worms
  • 05:43Results: Comparisons of Worm Gut Microbiomes and Environmental Communities
  • 06:26Conclusion

Summary

Automatische Übersetzung

Kompostmikrokosmos bringer den mikrobielle mangfoldighed, der findes i naturen, ind i laboratoriet for at lette mikrobiomforskning i Caenorhabditis elegans. Forudsat her er protokoller til opsætning af mikrokosmoseksperimenter, hvor eksperimenterne demonstrerer evnen til at modulere miljømikrobiel mangfoldighed for at udforske forholdet mellem miljømikrobiel mangfoldighed og ormgutmikrobiomsammensætning.

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