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Combining Clearing and Fluorescence Microscopy for Visualising Changes in Gene Expression and Physiological Responses to Plasmodiophora brassicae
 

Combining Clearing and Fluorescence Microscopy for Visualising Changes in Gene Expression and Physiological Responses to Plasmodiophora brassicae

Article DOI: 10.3791/64297-v • 06:58 min • August 5th, 2022
August 5th, 2022 •
1, 1, 1, 2, 3, 3, 1
1Integrative Plant Biology Team, Institute of Plant Genetics Polish Academy of Sciences, 2ZMBP-Center for Plant Molecular Biology, University of Tübingen, 3Department of General Botany, Institute of Experimental Biology, Faculty of Biology, Adam Mickiewicz University

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Summary
Automatic Translation

The present protocol describes an optimized method for the histological observation of galls induced by Plasmodiophora brassicae. Vibratome sections of hypocotyls are cleared before fluorescence imaging to study the involvement of transcription factors and phytohormones during disease progression. This protocol overcomes resin embedding limitations, enabling in planta visualization of fluorescent proteins.

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Clearing Microscopy Fluorescence Microscopy Gene Expression Physiological Responses Plasmodiophora Brassicae Clubroot Disease Galls Development Plant-pathogen Interaction Imaging Protocol Gene Expression Visualization Physiological Response Visualization Phytohormone Balance Disease Progression Tracking Spore Distribution Tracking Local Lignification Tracking Fluorescent Protein Preservation
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