Presented here are methods for preparing active nematics from microtubules and kinesin motors, including protein preparation and construction and the use of wells for active nematic confinement.
Memarian, F. L., Khaladj, D. A., Hammar, D., Hirst, L. S. Forming, Confining, and Observing Microtubule-Based Active Nematics. J. Vis. Exp. (191), e64287, doi:10.3791/64287 (2023).