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Organotypiske Retinal Explant Kulturer fra Macaque Monkey
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JoVE Journal Neurowissenschaften
Organotypic Retinal Explant Cultures from Macaque Monkey

Organotypiske Retinal Explant Kulturer fra Macaque Monkey

DOI:
10.3791/64178-v

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10:32 min

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August 24, 2022

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, , , , ,
1Yunnan Eye Institute & Key Laboratory of Yunnan Province, Yunnan Eye Disease Clinical Medical Center, Affiliated Hospital of Yunnan University,Yunnan University, 2Institute for Ophthalmic Research,Eberhard-Karls-Universität Tübingen

Kapitel

  • 00:04Introduction
  • 01:05Preparation of Retinal Explants
  • 03:08Primate Retinal Explant Culture
  • 03:36Fixing and Cryo‐Sectioning
  • 05:01Cyclic Guanosine Monophosphate (cGMP) Staining
  • 06:13TUNEL Staining
  • 07:58cGMP Staining Combined with TUNEL Staining
  • 08:40Results: Macaque Monkey Retinal Explant Culture
  • 09:20Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

Retinal explants hentet fra villtype makaker ble dyrket in vitro. Netthinnegenerasjon og cGMP-PKG-signalveien ble indusert ved bruk av PDE6-hemmeren zaprinast. cGMP-akkumulering i eksplantene ved forskjellige zaprinastkonsentrasjoner ble verifisert ved bruk av immunfluorescens.

Tags

Keywords: Organotypic Retinal ExplantMacaque MonkeyRetinal DegenerationIn Vivo ConditionsAnimal Use ReductionRetinal DevelopmentRetinal Explant PreparationBacterial ContaminationRetinal Explant CultureDrug TreatmentCryo-sectioningParaformaldehyde Fixation

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